Process Engineering · Proprietary Technology

Solid-State Fermentation
A Proprietary Processing Approach

AENON is developing a proprietary solid-state fermentation (SSF) process to transform red seaweed substrates into a bioactive feed additive for enteric methane reduction in ruminants.

Solid-State Fermentation Proprietary Process Bromoform-Free
Process Overview

What is Solid-State Fermentation?

Solid-State Fermentation (SSF) is a fermentation process conducted on a solid substrate with controlled moisture — as opposed to submerged liquid fermentation where the substrate is dissolved in water.

SSF has a long history in traditional fermented foods — miso, koji, tempeh — and is widely used in industrial enzyme, feed, and bioactive compound production.

For seaweed-based feed applications, AENON is developing SSF as the core processing pathway, chosen for its compatibility with red seaweed substrates and the bioactive compound profiles it produces.

🌾
Solid Substrate
Seaweed in dried form with controlled moisture addition
🧫
Microbial Fermentation
Selected microbial strains transform the substrate
⚗️
Bioactive Output
Sulphated polysaccharides and fermentation metabolites
📦
Dry Powder Product
Feed-compatible, no cold chain required
Process Rationale

Why Solid-State Fermentation
for Seaweed?

Red seaweed species contain distinctive polysaccharide fractions that present specific challenges for conventional fermentation approaches. AENON's SSF process is designed to work with these properties rather than against them.

🌊
Substrate Compatibility

The SSF process is designed for compatibility with the physical and chemical properties of red seaweed, maintaining substrate integrity throughout fermentation.

⚗️
Bioactive Preservation

SSF conditions support the production and preservation of the bioactive sulphated polysaccharide fractions responsible for the observed methane-reducing activity.

🏭
Industrial Scalability

Solid-state fermentation infrastructure is well-established in the feed ingredient industry, supporting a scalable production pathway.

📦
Feed-Compatible Output

The dried SSF product is designed for direct blending into total mixed rations (TMR) or compound feed premix without protocol changes at the farm.

Process Flow

From Seaweed to Feed Additive

🌊
Step 1 — Raw Material Selection
Red seaweed species · proprietary blend
Two complementary tropical red seaweed species are sourced and quality-screened. Both are commercially cultivated in Vietnam and are bromoform-free by nature. The blend formulation is proprietary.
⚙️
Step 2 — Substrate Preparation
Particle size · moisture standardisation
The dried seaweed blend is milled to a controlled particle size to ensure uniform microbial colonisation throughout the solid matrix. Moisture is standardised to support microbial activity — a critical balance in solid-state fermentation.
🧫
Step 3 — Microbial Inoculation
Proprietary microbial strains · co-culture
Selected microbial strains are inoculated into the prepared substrate. The strain selection and co-culture configuration are proprietary and chosen for compatibility with the seaweed substrate and target bioactive output. Detailed process parameters are held as proprietary technical information.
🌡️
Step 4 — Solid-State Fermentation
Controlled conditions · proprietary parameters
The inoculated substrate undergoes solid-state fermentation under controlled conditions optimised for target bioactive compound production. Specific process parameters — including temperature, duration, and environmental conditions — are maintained as proprietary technical information and available to qualified partners under NDA.
🔥
Step 5 — Drying & Stabilisation
Low moisture · storage-stable product
After fermentation, the substrate is dried to halt the process, inactivate viable microorganisms, and stabilise the product for feed supply chain handling. Drying conditions are optimised to preserve the bioactive compound fraction.
📦
Step 6 — Milling, QC & Packaging
SeaTrace™ SC-1 · feed-grade specification
The dried fermented substrate is milled to a feed-compatible particle size and subjected to quality control testing. SeaTrace™ SC-1 is packaged under moisture-barrier conditions for standard feed supply chain storage. It is designed for direct incorporation into TMR (Total Mixed Ration) systems without additional processing at the farm.
Technology Differentiation

What the SSF Process Enables

Bioactive Compound Production

SSF processing transforms the seaweed substrate and activates sulphated polysaccharide fractions that would not be as available from unprocessed seaweed alone.

Fermentation metabolites generated during SSF contribute to the indirect mechanism of methane reduction — redirecting metabolic hydrogen toward propionate synthesis in the rumen.

SSF Process Produces
  • Sulphated polysaccharide fragments (Direct Mechanism)
  • Short-chain organic acid metabolites (Indirect Mechanism)
  • Enhanced bioavailability of cell wall bioactives
  • Stable dry powder ready for feed integration
Enabling in the Rumen
  • Methanogenic archaea inhibition
  • Propionate pathway activation
  • ~50% CH₄ reduction observed in-vitro
  • Total VFA maintained (no digestion suppression)

In-vitro results are preliminary and do not guarantee equivalent in-vivo performance. Animal validation is planned for Q4 2026.

Technical Collaboration

Technical Information for Partners

Detailed process parameters, strain information, formulation data, and technical documentation are available to qualified industry partners and researchers under a non-disclosure agreement (NDA).

If you represent a feed manufacturer, livestock producer, or research institution and wish to explore technical collaboration, please contact us directly.

Technical Partnership Inquiry Dual Mechanism →